Exosome Subclass Analysis
Exosomes are extracellular vesicles that have attracted attention since the late 2000s as communication agents between cells, as well as potential diagnostic/therapeutic tools for various disorders. Much effort has been put into research on their secretion/ transport modality, the substance contained within and other characteristics. The reality, however, is that there is no consensus on the fundamental question, "What are exosomes?" In order to define exosomes, research on their isolation method is essential.
Dr. Kiyotaka Shiba conducts research focusing on exosome and leads the Protein Engineering Division at the Cancer Institute of the Japanese Foundation for Cancer Research. Along with research scholars at the institute, Dr. Kazuya Iwai and Dr. Satoshi Yamamoto, from the Department of Oral Implantology at the Tokyo Dental College, Dr. Shiba discusses subclass analysis based on exosome density, as well as purification methods and cautions.