Bead Size Selection

Sample to Bead Ratio for Size Selection Matters

Not all bead based cleanup kits use the 1.8x ratio; the figure below shows that of 10 bead-based cleanup kits only 3 suggest a 1.8x ratio for cleanup.

Bead Ratio and Comparison:

Bead Size Selection Ratio

 

The Impact of Switching to a Kit with a Different Ratio

  • Cleanups will require a different bead ratio that could lead to loss of fragments of interest
  • Sample to bead ratios will need to be recalculated
  • Lab protocols that use the standard sample to bead ratios that of AMPure XP or SPRIselect will have to be re-written

SPRI Methodology Video

More on AMPure XP

More on SPRIselect

 

Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Purchase AMPure XP

AMPure 5

AMPure XP, 5 mL
A63880
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AMPure 60

AMPure XP, 60 mL
A63881
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AMPure 450

AMPure XP, 450 mL
A63882
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Purchase SPRIselect

 SPRISelect Reagent for DNA/RNA Size Selection and Cleanup

SPRIselect, 5 mL
B23317
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 SPRISelect Reagent for DNA/RNA Size Selection and Cleanup

SPRIselect, 60 mL
B23318
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 SPRISelect Reagent for DNA/RNA Size Selection and Cleanup

SPRIselect, 450 mL
B23319
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