RNAClean XP Performance and Data
RNAClean XP provides a simple, flexible and highly reproducible method for purifying RNA and nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions. This method utilizes the Solid Phase Reversible Immobilization (SPRI) magnetic bead-based technology. It is uniquely formatted for purification of both the cDNA and RNA steps in NGS based procedures. This technique is easily performed manually in far less time than competitive methods. The RNAClean XP system doesn’t use organic solvents, vacuum filtration, or centrifugation, and delivers superior nucleic acid recovery and purity for use in downstream applications.
- Purification of cDNA and RNA
- Complete removal of salts, unincorporated primers and dNTPs
- Simple automation-friendly protocol with no centrifugation, filtration or precipitation steps
Starting Concentration and Percent Yield
Three starting concentrations were purified using RNAClean XP. The average for each starting concentration is shown in the graph. The three starting concentrations all had a percent yield that was similar. The average recovery from the three cleanup experiments was 78% with a σ2 of 10%.
- Bind RNA to magnetic beads
- Separate beads from contaminants
- Wash magnetic beads with 70% ethanol to remove contaminants
- Elute RNA from magnetic beads
- Transfer to new plate
Products to Clean RNA & cDNA
|A63987||RNAClean XP Kit||40 mL|
|A66514||RNAClean XP Kit||450 mL|
Not intended or validated for use in the diagnosis of disease or other conditions.
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